- Title
- A Pseudoalteromonas Clade with remarkable biosynthetic potential
- Creator
- Chau, Rocky; Pearson, Leanne A.; Cain, Jesse; Kalaitzis, John A.; Neilan, Brett A.
- Relation
- Applied and Environmental Microbiology Vol. 87, Issue 6, no. e02604-20
- Publisher Link
- http://dx.doi.org/10.1128/AEM.02604-20
- Publisher
- American Society for Microbiology
- Resource Type
- journal article
- Date
- 2021
- Description
- Pseudoalteromonas species produce a diverse range of biologically active compounds, including those biosynthesized by nonribosomal peptide synthetases (NRPSs) and polyketide synthases (PKSs). Here, we report the biochemical and genomic analysis of Pseudoalteromonas sp. strain HM-SA03, isolated from the blue-ringed octopus, Hapalochlaena sp. Genome mining for secondary metabolite pathways revealed seven putative NRPS/PKS biosynthesis gene clusters, including those for the biosynthesis of alterochromides, pseudoalterobactins, alteramides, and four novel compounds. Among these was a novel siderophore biosynthesis gene cluster with unprecedented architecture (NRPS-PKS-NRPS-PKS-NRPS-PKS-NRPS). Alterochromide production in HM-SA03 was also confirmed by liquid chromatography-mass spectrometry. An investigation of the biosynthetic potential of 42 publicly available Pseudoalteromonas genomes indicated that some of these gene clusters are distributed throughout the genus. Through the phylogenetic analysis, a particular subset of strains formed a clade with extraordinary biosynthetic potential, with an average density of 10 biosynthesis gene clusters per genome. In contrast, the majority of Pseudoalteromonas strains outside this clade contained an average of three clusters encoding complex biosynthesis. These results highlight the underexplored potential of Pseudoalteromonas as a source of new natural products.
- Subject
- alteromonas; antiSMASH; bacteriocin; genome; lanthipeptide; nonribosomal peptide
- Identifier
- http://hdl.handle.net/1959.13/1436623
- Identifier
- uon:40084
- Identifier
- ISSN:0099-2240
- Language
- eng
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